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Product Name
Anti-Phospho-c-Myc (Ser62) Rabbit antibody
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Description
Phospho-c-Myc (Ser62) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
MRTL; MYCC; c-Myc; bHLHe39 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser62 of human c-Myc
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/200
FC: 1/20
IP: 1/20
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Validations
Immunocytochemistry of c-Myc (Phospho-Ser62)(green) in Hela cells using c-Myc (Phospho-Ser62) Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of c-Myc (Phospho-Ser62) in 3t3 cell lysates using c-Myc (Phospho-Ser62) Rabbit pAb(1:1000 diluted).Predicted band size:49KDa.Observed band size:57KDa.
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Background
Swiss-Prot Acc.P01106.Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"