Anti-PHD1/prolyl hydroxylase Rabbit antibody

Cat.#: 168031

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Product Information

  • Product Name
    Anti-PHD1/prolyl hydroxylase Rabbit antibody
  • Documents
  • Description
    PHD1/prolyl hydroxylase Rabbit monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthesized peptide derived from human PHD1/prolyl hydroxylase
  • Clonality
    Monoclonal
  • Formulation
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500-1/2000

    IHC: 1/50-1/200

    ICC/IF: 1/50-1/200

    FC: 1/50

  • Validations

    Western blot detection of PHD1/prolyl hydroxylase in Hela,A549,HL-60,U2OS,U87-MG cell lysates using PHD1/prolyl hydroxylase Rabbit mAb(1:1000 diluted).Observed band size:44kDa.

    Western blot detection of PHD1/prolyl hydroxylase in Hela,A549,HL-60,U2OS,U87-MG cell lysates using PHD1/prolyl hydroxylase Rabbit mAb(1:1000 diluted).Observed band size:44kDa.

  • Background
    Swiss-Prot Acc.Q96KS0.Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"