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Product Name
Anti-PHD1/prolyl hydroxylase Rabbit antibody
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Description
PHD1/prolyl hydroxylase Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: A synthesized peptide derived from human PHD1/prolyl hydroxylase
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Clonality
Monoclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/50
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Validations
Western blot detection of PHD1/prolyl hydroxylase in Hela,A549,HL-60,U2OS,U87-MG cell lysates using PHD1/prolyl hydroxylase Rabbit mAb(1:1000 diluted).Observed band size:44kDa.
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Background
Swiss-Prot Acc.Q96KS0.Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"