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Product Name
Anti-Peroxiredoxin 1/PAG (6A6) Mouse antibody
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Description
Peroxiredoxin 1/PAG (6A6) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Recombinant Protein of Peroxiredoxin-1
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-3000
IF: 1/100-200
IHC: 1/50-300
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Validations
Western blot detection of Prdx 1 in T47D,3T3,COS7,C6 and Hela cell lysates using Prdx 1 mouse mAb (1:3000 diluted).Predicted band size:21kDa.Observed band size:21kDa.
Western blot detection of Peroxiredoxin 1/Prdx1 in Jurkat,CHO-K1,C6,Hela cell lysates using Peroxiredoxin 1/Prdx1 (6A6) Mouse mAb(1:1000 diluted).Predicted band size:21KDa.Observed band size:21KDa.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Western blot analysis of 1) MCF7, 2) Rat Kidney Tissue, 3) Mouse Brain Tissue, diluted at 1:2000.
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Background
Swiss-Prot Acc.Q06830.Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"