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Product Name
Anti-Pepsinogen C/PGC antibody
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Description
Mouse monoclonal to Pepsinogen C/PGC
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Tested applications
ELISA, IHC-P
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Species reactivity
Human Pepsinogen C / PGC
No cross-reactivity in WB and ELISA with Human cell lysate (293 cell line) -
Alternative names
2210410L06Rik antibody; FLJ99563 antibody; gastricsin antibody; gastricsin antibody; MGC117575 antibody; Pepsinogen C antibody; PG1 antibody; Pg-1 antibody; Pgc antibody; PGC antibody; PGII antibody; preprogastricsin antibody; progastricsin antibody; RP11-298J23.1 antibody; Upg1 antibody; PEPC antibody; pepsin C antibody; Pepsinogen C antibody; pepsinogen group II antibody; progastricsin antibody; Upg1 antibody; Upg-1 antibody; urinary pepsinogen 1 antibody; PGII antibody; PEPC antibody; Upg1 antibody; Upg-1 antibody; 2210410L06Rik antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Pepsinogen C / PGC (rh Pepsinogen C / PGC; NP_002621.1; Met 1-Ala 388). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PGC. The detection limit for Human PGC is approximately 0.31 ng/well.
IHC-P: 5-25 μg/mL
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Validations
Pepsinogen C / PGC Antibody, Mouse MAb, Immunohistochemistry
Immunochemical staining of human PGC in human stomach (from 3 donors) with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
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References
- Richter C, et al. (1998) Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin. Biochem J. 1 (335): 481-90.
- Westerveld BD, et al. (1987) Gastric proteases in Barrett's esophagus. Gastroenterology. 93 (4): 774-8.
- Ichinose M, et al. (1991) Methylation and expression of human pepsinogen genes in normal tissues and their alteration in stomach cancer. Jpn J Cancer Res. 82 (6): 686-92.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"