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Product Name
Anti-PDLIM1/CLP36 Rabbit antibody
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Description
PDLIM1/CLP36 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PDLIM1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/50
FC: 1/20-1/100
IP: 1/20
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Validations
Western blot detection of PDLIM1 in K562,C6,3T3,Hela cell lysates using PDLIM1 Rabbit pAb(1:1000 diluted).Predicted band size:36kDa.Observed band size:36kDa.
Immunocytochemistry of PDLIM1(green) in Hela cells using PDLIM1 Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.O00151.Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"