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Product Name
Anti-PDK2 Mouse antibody
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Description
PDK2 Mouse monoclonal antibody
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Tested applications
WB, FC
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Species reactivity
Human
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of A431 cells using PDK2 mouse mAb (green) and negative control (red).
Western blot detection of PDK2 in A549,MCF7,C6 and Hela cell lysates using PDK2 mouse mAb (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
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Background
Swiss-Prot Acc.Q15119.This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"