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Product Name
Anti-PCSK9 antibody
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Description
Mouse monoclonal to PCSK9
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Tested applications
IHC-P
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Species reactivity
Rat PCSK9
No cross-reactivity in ELISA with Human PCSK9; Rhesus PCSK9 -
Alternative names
AI415265 antibody; AI747682 antibody; FH3 antibody; FH3 antibody; HCHOLA3 antibody; HCHOLA3 antibody; LDLCQ1 antibody; Macaca mulatta antibody; MGC47409 antibody; NARC1 antibody; Narc1 antibody; Narc1 antibody; NARC-1 antibody; NARC-1 antibody; PC9 antibody; PC9 antibody; PC9 antibody; PC9 antibody; Pcsk9 antibody; Pcsk9 antibody; PCSK9 antibody; Pcsk9 antibody; PSEC0052 antibody; RP23-159J7.1 antibody; FH3 antibody; PC9 antibody; NARC1 antibody; LDLCQ1 antibody; NARC-1 antibody; HCHOLA3 antibody; FH3 antibody; PC9 antibody; Narc1 antibody; HCHOLA3 antibody; AI415265 antibody; AI747682 antibody
- Immunogen
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat PCSK9 (rR PCSK9; NP_954862.2; Met1-Gln691). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 10-30 μg/mL
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Validations
PCSK9 Antibody, Mouse MAb, Immunohistochemistry
Immunochemical staining of rat PCSK9 in rat brain with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons. References
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References
- Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
- Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
- Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
- Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
- Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"