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Product Name
Anti-PCNA antibody
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Description
Rabbit polyclonal to PCNA
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Tested applications
WB, IHC-P, IF, IP, ICC/IF
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Species reactivity
Human PCNA
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Alternative names
MGC8367 antibody; RP23-33N15.1 antibody; ATLD2 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PCNA, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-10 μg/ml
IHC-P: 0.1-2 μg/mL
ICC/IF: 0.5-1.5 μg/mL
IP: 0.5-2 uL/mg of lysate
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Validations
PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human PCNA in human ovarian cancer with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human PCNA in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of PCNA in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human PCNA polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Go
PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Proliferating Cell Nuclear Antigen (PCNA) is a protein only expresse in nomal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
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References
- Balajee AS, et al. (2001) Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acids Res. 29 (6): 1341-51.
- Ducoux M, et al. (2001) Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta. J Biol Chem. 276 (52): 49258-66.
- Tetsuo I, et al. (2002) PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 7(10): 997-1007.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"