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  • Anti-PCNA (9C9) Mouse antibody
  • Anti-PCNA (9C9) Mouse antibody
  • Anti-PCNA (9C9) Mouse antibody
  • Anti-PCNA (9C9) Mouse antibody
  • Anti-PCNA (9C9) Mouse antibody

Anti-PCNA (9C9) Mouse antibody

Cat.#: 169060

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Special Price 1354.8 USD

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Product Information

  • Product Name
    Anti-PCNA (9C9) Mouse antibody
  • Documents
  • Description
    PCNA (9C9) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IHC-P
  • Species reactivity
    Human, Rat, Mouse
  • Isotype
    IgG1
  • Preparation
    Antigen: Synthetic Peptide of PCNA
  • Clonality
    Monoclonal antibody
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/5000

    IHC: 1/200

    IF: 1/200

  • Validations

    IHC staining of Human breast cancer tissue paraffin-embedded, diluted at 1:200

    IHC staining of Human breast cancer tissue paraffin-embedded, diluted at 1:200

    Western blot detection of PCNA in Jurkat,CHO-K1,3T3,Hela cell lysates using PCNA (9C9) Mouse mAb(1:1000 diluted).Predicted band size:29KDa.Observed band size:34KDa.

    Western blot detection of PCNA in Jurkat,CHO-K1,3T3,Hela cell lysates using PCNA (9C9) Mouse mAb(1:1000 diluted).Predicted band size:29KDa.Observed band size:34KDa.

    Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4), diluted at 1:5000.

    Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4), diluted at 1:5000.

    The picture was kindly provided by our customer

    The picture was kindly provided by our customer

    Western blot detection of PCNA in T47D,3T3 and COS7 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

    Western blot detection of PCNA in T47D,3T3 and COS7 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

  • Background
    Swiss-Prot Acc.P12004.Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"