Anti-PCNA (9C9) Mouse antibody
PCNA (9C9) Mouse monoclonal antibody
WB, ICC/IF, IHC-P
Human, Rat, Mouse
Antigen: Synthetic Peptide of PCNA
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
IHC staining of Human breast cancer tissue paraffin-embedded, diluted at 1:200
Western blot detection of PCNA in Jurkat,CHO-K1,3T3,Hela cell lysates using PCNA (9C9) Mouse mAb(1:1000 diluted).Predicted band size:29KDa.Observed band size:34KDa.
Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4), diluted at 1:5000.
The picture was kindly provided by our customer
Western blot detection of PCNA in T47D,3T3 and COS7 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
Swiss-Prot Acc.P12004.Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
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