Anti-PBK/SPK Mouse antibody
PBK/SPK Mouse monoclonal antibody
WB, IHC-P, ICC/IF, FC
Antigen: Purified recombinant fragment of human PBK expressed in E. Coli.
Ascitic fluid containing 0.03% sodium azide.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot detection of PBK/TOPK in Jurkat,A549,MCF7,C6 and Hela cell lysates using PBK/TOPK mouse mAb (1:5000 diluted).Predicted band size:36KDa.Observed band size:40KDa.
Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.
Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.
Swiss-Prot Acc.Q96KB5.This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
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