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  • Anti-PBK/SPK Mouse antibody
  • Anti-PBK/SPK Mouse antibody
  • Anti-PBK/SPK Mouse antibody
  • Anti-PBK/SPK Mouse antibody
  • Anti-PBK/SPK Mouse antibody
  • Anti-PBK/SPK Mouse antibody

Anti-PBK/SPK Mouse antibody

Cat.#: 168546

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-PBK/SPK Mouse antibody
  • Documents
  • Description
    PBK/SPK Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant fragment of human PBK expressed in E. Coli.
  • Clonality
    Monoclonal antibody
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

    Western blot detection of PBK/TOPK in Jurkat,A549,MCF7,C6 and Hela cell lysates using PBK/TOPK mouse mAb (1:5000 diluted).Predicted band size:36KDa.Observed band size:40KDa.

    Western blot detection of PBK/TOPK in Jurkat,A549,MCF7,C6 and Hela cell lysates using PBK/TOPK mouse mAb (1:5000 diluted).Predicted band size:36KDa.Observed band size:40KDa.

    Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).

    Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).

    Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.

    Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.

    Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.

  • Background
    Swiss-Prot Acc.Q96KB5.This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"