Anti-PARP-1/PARP antibody

Anti-PARP-1/PARP antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Rabbit polyclonal to PARP-1/PARP

WB, IHC-P, IF, IP, ICC/IF

Human PARP-1 / PARP

Adprt1 antibody; ADPRT1 antibody; AI893648 antibody; C80510 antibody; pADPRT-1 antibody; PARP antibody; PARP antibody; Parp1 antibody; PARP1 antibody; parp-1 antibody; PARP-1 antibody; PPOL antibody; PPOL antibody; RP11-125A15.2 antibody; sPARP-1 antibody; PARP antibody; PPOL antibody; ADPRT antibody; ARTD1 antibody; ADPRT1 antibody; PARP-1 antibody; pADPRT-1 antibody; PARP antibody; PPOL antibody; ARTD1 antibody; Adprp antibody; Adprt1 antibody; C80510 antibody; parp-1 antibody; sPARP-1 antibody; AI893648 antibody; 5830444G22Rik antibody; 5830444G22Rik antibody; Adprp antibody; ADPRT antibody

Rabbit IgG

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PARP-1 / PARP, and purified by antigen affinity chromatography.

Polyclonal

0.2 μm filtered solution in PBS

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

• Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
• Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
• Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
• Ahell I. et al., 2008, Nature. 451: 81-85.