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Anti-P2RY8 antibody

Cat.#: 175629

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-P2RY8 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to P2RY8
  • Tested applications
    WB, FC
  • Species reactivity
    Human
  • Alternative names
    P2Y8 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human p2y8 131-230/359
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:2ug/Test

  • Validations

    Fig1: Sample:; Raji Cell (Human) Lysate at 30 ug; Primary: Anti- P2Y8 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 66 kD; Observed band size: 50 kD

    Fig1: Sample:; Raji Cell (Human) Lysate at 30 ug; Primary: Anti- P2Y8 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 66 kD; Observed band size: 50 kD

    Fig2: Blank control: HL60.; Primary Antibody (green line): Rabbit Anti-P2Y8 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig2: Blank control: HL60.; Primary Antibody (green line): Rabbit Anti-P2Y8 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig3: Blank control: Raji.; Primary Antibody (green line): Rabbit Anti-P2Y8 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-AF488; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig3: Blank control: Raji.; Primary Antibody (green line): Rabbit Anti-P2Y8 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-AF488; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  • Background
    Q86VZ1

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"