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Product Name
Anti-OTX2 (3C11) Mouse antibody
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Description
OTX2 (3C11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human OTX2 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
Immunohistochemical analysis of paraffin-embedded stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.
Immunofluorescence analysis of U251 cells using OTX2 mouse mAb (green). Red
Western blot detection of OTX2 in C6,Jurkat,K562 and 3T3 cell lysates using OTX2 mouse mAb (1:500 diluted).Predicted band size:32KDa.Observed band size:40KDa.
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Background
Swiss-Prot Acc.P32243.This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development.Tissue specificity: Expressed in brain.
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