Anti-OSBPL1A antibody

Fig1:; Western blot analysis of ORP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% NFTM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Mouse heart tissue lysate; Lane 2: Rat brain tissue lysate; Lane 3: A549 cell lysate; Lane 4: PC-3 cell lysate

Fig2:; Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ORP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.