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Product Name
Anti-OLIG1 antibody
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Description
Rabbit polyclonal to OLIG1
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Tested applications
WB, IF, IP, ICC/IF
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Species reactivity
Human OLIG1
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human OLIG1, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
ICC/IF: 0.5-1.5 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
OLIG1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
OLIG1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of OLIG1 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OLIG1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
OLIG1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Immunochemical staining of human TROP2 in human prostate with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"