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Product Name
Anti-Oct4 / POU5F1 antibody
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Description
Mouse monoclonal to Oct4 / POU5F1
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Tested applications
FCM
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Species reactivity
Human Oct4
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Alternative names
42280 antibody; 42281 antibody; Otf3 antibody; Otf4 antibody; NF-A3 antibody; Otf-3 antibody; Otf-4 antibody; Otf3g antibody; Oct3/4 antibody; Oct-3/4 antibody; Otf3-rs7 antibody; 42280 antibody; 42281 antibody; OTF3 antibody; OTF4 antibody; OTF-3 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Oct4, and it was conjugated with FITC under optimum conditions, the unreacted FITC was removed.
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Clonality
Monoclonal
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Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
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Storage instructions
This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. -
Applications
FCM
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Validations
Oct4 Antibody (FITC), Mouse MAb, Flow cytometric
Flow cytometric analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer (BD Pharmingen™ Cat. No. 554714), and stained with FITC Mouse anti-Oct4 monoclonal antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"