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Product Name
Anti-Oct4 / POU5F1 antibody
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Description
Mouse monoclonal to Oct4 / POU5F1
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Tested applications
FCM
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Species reactivity
Human Oct4
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Alternative names
42280 antibody; 42281 antibody; Otf3 antibody; Otf4 antibody; NF-A3 antibody; Otf-3 antibody; Otf-4 antibody; Otf3g antibody; Oct3/4 antibody; Oct-3/4 antibody; Otf3-rs7 antibody; 42280 antibody; 42281 antibody; OTF3 antibody; OTF4 antibody; OTF-3 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Oct4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
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Validations
Oct4 Antibody, Mouse MAb, Flow Cytometry
Flow cytometric analysis of Oct4 in human embryonic stem (ES) cells. Human embryonic stem (ES) cells were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization buffer (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-Oct4 monoclonal antibody (1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"