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Product Name
Anti-Nrf2 (5F10) Mouse antibody
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Description
Nrf2 (5F10) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human NFE2L2 (AA: 356-589) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Western blot analysis of extracts from Jurkat,A549,MCF7,C6 and Hela cell lysates using NRF2 mouse mAb (1:2000 diluted).Predicted band size:68KDa.Observed band size:68KDa.
Western blot analysis using NFE2L2 mAb against human NFE2L2 (AA: 356-589) recombinant protein. (Expected MW is 52.1 kDa)
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using NFE2L2 mouse mAb with DAB staining.
Western blot analysis of extracts from Hela and MCF7 cells using NRF2 Mouse mAb at 1:1000 dilution. Predicted band size: 68KDa.Observed band size: 68KDa.
Western blot analysis using NFE2L2 mAb against HEK293 (1) and NFE2L2 (AA: 356-589)-hIgGFc transfected HEK293 (2) cell lysate.
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Background
Swiss-Prot Acc.Q16236.This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"