Anti-NRCAM antibody

Cat.#: 175134

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Product Information

  • Product Name
    Anti-NRCAM antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to NRCAM
  • Tested applications
    WB, ICC, IHC-P
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein within human nrcam aa 700-900.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:50-1:200

    IHC-P: 1:50 -1:200

  • Validations

    Fig1: Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.

    Fig2: ICC staining NRCAM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining NRCAM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining NRCAM in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining NRCAM in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining NRCAM in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining NRCAM in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.

  • Background
  • References
    • Kwon H S et al. Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling. Biol Chem 288:26357-26371(2013).
    • Lane R P et al. Characterization of a highly conserved human homolog to the chicken neural cell surface protein Bravo/Nr-CAM that maps to chromosome band 7q31. Genomics 35:456-465(1996).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"