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Product Name
Anti-NOLC1 Rabbit antibody
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Description
NOLC1 Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse
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Alternative names
P130; NOPP130; NOPP140; NS5ATP13 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human NOLC1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
IHC: 1/20
ICC/IF: 1/100
FC: 1/50
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Validations
Western blot detection of NOLC1 in Jurkat cell lysates using NOLC1 Rabbit mAb(1:500 diluted).Predicted band size:74kDa.Observed band size:110kDa.
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Background
Swiss-Prot Acc.Q14978.Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).
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