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Product Name
Anti-NFI-C/CTF (4E10) Mouse antibody
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Description
NFI-C/CTF (4E10) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
1110019L22Rik;1500041O16Rik;AA589446;AI746521;CAAT box transcription factor;CCAAT binding transcription factor;CCAAT box binding transcription factor;CCAAT-box-binding transcription factor;CNFI C;CTF;CTF5;MGC137374;MGC20153;NF I;NF I/C;NF-I/C;NF1 C;NF1-C; antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human NFIC protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Ascites containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/200
;ICC: 1/200
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Validations
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).
Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.
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Background
Swiss-Prot Acc.P08651.Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"