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Product Name
Anti-NFASC / Neurofascin antibody
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Description
Mouse monoclonal to NFASC / Neurofascin
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Tested applications
FCM
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Species reactivity
Human NFASC / Neurofascin
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Alternative names
Neurofascin antibody; NFASC antibody; RP11-494K3.1 antibody; NF antibody; NRCAML antibody; neurofascin homolog antibody; Neurofascin antibody; Nfasc antibody; AA387016 antibody; D430023G06Rik antibody; NF antibody; mKIAA0756 antibody; NF antibody; NRCAML antibody; NF antibody; AA387016 antibody; mKIAA0756 antibody; D430023G06Rik antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NFASC / Neurofascin (rh NFASC / Neurofascin; O94856-12; Met1-Gln939) and conjugated with PE under optimum conditions, the unreacted PE was removed.
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Clonality
Monoclonal
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Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
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Storage instructions
This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. -
Applications
FCM
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Validations
NFASC / Neurofascin Antibody (PE), Mouse MAb, Flow Cytometry
Flow cytometric analysis of Human NFASC expression on SH-SY5Y cells. Cells were stained with PE-conjugated anti-Human NFASC. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Background
NFASC, also known as neurofascin, belongs to the immunoglobulin superfamily, L1/neurofascin/NgCAM family. It contains 5 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. NFASC functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. NFASC links the AIS extracellular matrix to the intracellular cytoskeleton.
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References
- Volkmer H. et al., 1992, J Cell Biol. 118 (1): 149-61.
- Burmeister M. et al., 1996, Mamm Genome. 7 (7): 558-9.
- Ango F. et al., 2004, Cell. 119 (2): 257-72.
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