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Anti-NELFA antibody

Cat.#: 175055

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Product Information

  • Product Name
    Anti-NELFA antibody
  • Documents
  • Description
    Mouse monoclonal antibody to NELFA
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Rat
  • Alternative names
    WHSC2 antibody; NELF-A antibody; P/OKcl.15 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:50-1:200

    IHC-P: 1:100-1:200

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of WHSC2 on HEK293 (1) and WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of WHSC2 on HEK293 (1) and WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.

    Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.; Positive control:; Line1:Jurkat Line2:HeLa Line3:HEK293; Line4:Rat brain Line5:A549 Line6:SPC-A-1

    Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.; Positive control:; Line1:Jurkat Line2:HeLa Line3:HEK293; Line4:Rat brain Line5:A549 Line6:SPC-A-1

    Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.

    Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

    Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

  • Background
    Q9H3P2
  • References
    • Kerzendorfer C et al. Characterizing the functional consequences of haploinsufficiency of NELF-A (WHSC2) and SLBP identifies novel cellular phenotypes in Wolf-Hirschhorn syndrome. Hum Mol Genet 21(10):2181-93 (2012).
    • Yung TM et al. Cellular dynamics of the negative transcription elongation factor NELF. Exp Cell Res 315(10):1693-705 (2009).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"