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Product Name
Anti-NDUFAF5 Rabbit antibody
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Description
NDUFAF5 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
C20orf7; dJ842G6.1; bA526K24.2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human NDUFAF5
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/200
ICC/IF: 1/100
FC: 1/50
IP: 1/20
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Validations
Immunohistochemistry of NDUFAF5 in paraffin-embedded Human breast cancer tissue using NDUFAF5 Rabbit pAb at dilution 1/50
Western blot detection of NDUFAF5 in 3T3 cell lysates using NDUFAF5 Rabbit pAb(1:1000 diluted).Predicted band size:39kDa.Observed band size:39kDa.
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Background
Swiss-Prot Acc.Q5TEU4.Arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages (PubMed:18940309, PubMed:27226634). Acts by mediating hydroxylation of 'Arg-111' of NDUFS7 (PubMed:27226634). May also have methyltransferase activity (Probable).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"