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Product Name
Anti-Nck beta Rabbit antibody
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Description
Nck beta Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GRB4; NCKbeta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Nck beta
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/5000
ICC/IF: 1/20-1/50
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Validations
Western blot detection of Nck beta in K562,Rat Brain,C6,3T3,Hela cell lysates using Nck beta Rabbit mAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.
Immunocytochemistry of Nck beta(green) in Hela cells using Nck beta Rabbit mAb at dilution 1/200, and DAPI(blue)
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Background
Swiss-Prot Acc.O43639.Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"