-
Product Name
Anti-NCF2 / NCF-2 / P67phox antibody
- Documents
-
Description
Rabbit polyclonal to NCF2 / NCF-2 / P67phox
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human NCF2 / NCF-2 / P67phox
-
Alternative names
neutrophil cytosolic factor 2 antibody; NCF2 antibody; RP1-127C7.5 antibody; NCF-2 antibody; NOXA2 antibody; P67-PHOX antibody; P67PHOX antibody; 67 kDa neutrophil oxidase factor antibody; NADPH oxidase activator 2 antibody; chronic granulomatous disease antibody; autosomal 2 antibody; neutrophil NADPH oxidase factor 2 antibody; neutrophil cytosol factor 2 antibody; neutrophil cytosolic factor 2 (65kD antibody; chronic granulomatous disease antibody; autosomal 2) antibody; neutrophil cytosolic factor 2 antibody; Ncf2 antibody; NOXA2 antibody; Ncf-2 antibody; p67phox antibody; 67 kDa neutrophil oxidase factor antibody; NADPH oxidase activator 2 antibody; NADPH oxidase subunit (67 kD) antibody; NADPH oxidase subunit (67kDa) antibody; neutrophil NADPH oxidase factor 2 antibody; neutrophil cytosol factor 2 antibody; p67-phox antibody; NCF-2 antibody; NOXA2 antibody; P67PHOX antibody; P67-PHOX antibody; NOXA2 antibody; Ncf-2 antibody; p67phox antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Human NCF2 / NCF-2 / P67phox (rh NCF2 / NCF-2 / P67phox; AAH01606.1; Met1-Val526). NCF2 / NCF-2 / P67phox specific IgG was purified by Human NCF2 / NCF-2 / P67phox affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NCF2 / NCF-2 / P67phox. The detection limit for Human NCF2 / NCF-2 / P67phox is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
-
Validations
NCF2 / NCF-2 / P67phox Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
NCF2 / NCF-2 / P67phox Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
-
Background
NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.
-
References
- Wientjes FB. et al., 1996, Semin Cell Biol. 6 (6): 357-65.
- DeLeo FR. et al., 1997, J Leukoc Biol. 60 (6): 677-91.
- Dorseuil O. et al., 1997, C R Seances Soc Biol Fil. 191 (2): 237-46.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"