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Product Name
Anti-NBL1/DAND1 antibody
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Description
Rabbit polyclonal to NBL1/DAND1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human NBL1 / DAN / DAND1
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Alternative names
NB antibody; DAN antibody; NO3 antibody; DAND1 antibody; D1S1733E antibody; DAN antibody; NO3 antibody; Dana antibody; D4H1S1733E antibody; D1S1733E antibody; D4H1S1733E antibody; DAN antibody; DAN antibody; Dana antibody; DAND1 antibody; MGC123430 antibody; MGC8972 antibody; Nbl1 antibody; NBL1 antibody; NO3 antibody; NO3 antibody; RP23-65B16.3 antibody; RP5-1056L3.4 antibody; NB antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human NBL1 / DAN (rh NBL1; NP_005371.1; Met 1-Asp 180). NBL1 specific IgG was purified by human NBL1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human DAN. The detection limit for Human DAN is 0.00245 ng/well.
IHC-P: 0.5-5 μg/mL
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Validations
NBL1 / DAND1 / DAN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human DAN in human hepatoma with rabbit polyclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
NBL1 / DAND1 / DAN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human DAN in human kidney with rabbit polyclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
NBL1 / DAND1 / DAN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human DAN in human lung with rabbit polyclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.
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References
- Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.
- Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.
- Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.
- Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"