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Anti-N WASP Rabbit antibody

Cat.#: 167240

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-N WASP Rabbit antibody
  • Documents
  • Description
    N WASP Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse
  • Alternative names
    NWASP; WASPB; N-WASP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human N-WASP
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/1000-1/2000

    IHC: 1/20-1/50

    ICC/IF: 1/50

    FC: 1/20-1/100

  • Validations

    Western blot detection of N-WASP in 3T3 cell lysates using N-WASP Rabbit pAb(1:1000 diluted).Predicted band size:55kDa.Observed band size:65kDa.

    Western blot detection of N-WASP in 3T3 cell lysates using N-WASP Rabbit pAb(1:1000 diluted).Predicted band size:55kDa.Observed band size:65kDa.

    Immunohistochemistry of N-WASP in paraffin-embedded Human colon cancer tissue using N-WASP Rabbit pAb at dilution 1/20

    Immunohistochemistry of N-WASP in paraffin-embedded Human colon cancer tissue using N-WASP Rabbit pAb at dilution 1/20

    Immunocytochemistry of N-WASP(green) in Hela cells using N-WASP Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of N-WASP(green) in Hela cells using N-WASP Rabbit pAb at dilution 1/50, and DAPI(blue)

  • Background
    Swiss-Prot Acc.O00401.Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:9422512, PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:9422512, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression . Plays a role in dendrite spine morphogenesis .

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"