-
Product Name
Anti-Myelin oligodendrocyte glycoprotein antibody
- Documents
-
Description
Rabbit polyclonal to Myelin oligodendrocyte glycoprotein
-
Tested applications
ELISA, WB, IHC-P
-
Species reactivity
Rat Myelin oligodendrocyte glycoprotein
-
Alternative names
BTN6 antibody; BTNL11 antibody; MOGIG2 antibody; NRCLP7 antibody; B230317G11Rik antibody; B230317G11Rik antibody; DAQB-92E24.2 antibody; MGC26137 antibody; Mog antibody; MOG antibody; MOGIG2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Rat Myelin oligodendrocyte glycoprotein (1PKQ_E; 1M-139H). Myelin oligodendrocyte glycoprotein specific IgG was purified by Rat Myelin oligodendrocyte glycoprotein affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat Myelin oligodendrocyte glycoprotein. The detection limit for Rat Myelin oligodendrocyte glycoprotein is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
-
Validations
Myelin oligodendrocyte glycoprotein Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Myelin oligodendrocyte glycoprotein Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of rat MOG in rat brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
Myelin oligodendrocyte glycoprotein (MOG) is a transmembrane protein belonging to immunoglobulin superfamily, and contains an Ig-like domain followed by two potential membrane-spanning regions. MOG is expressed only in the CNS with very low content (approximately 0.1% total proteins) in oligodendrogliocyte membrane. Three possible functions for MOG were suggested: (a) a cellular adhesive molecule, (b) a regulator of oligodendrocyte microtubule stability, and (c) a mediator of interactions between myelin and the immune system, in particular, the complement cascade. A direct interaction might exist between the membrane-associated regions of MOG and the myelin-specific glycolipid galactocerebroside (Gal-C), and such an interaction may have important consequences regarding the membrane topology and function of both molecules. It is considered that MOG is an autoantigen capable to produce a demyelinating multiple sclerosis-like disease in experimental animals.
-
References
- Chekhonin VP, et al. (2003) Myelin oligodendrogliocyte glycoprotein: the structure, functions, role in pathogenesis of demyelinating disorders. Biomed Khim. 49(5): 411-23.
- Hilton AA, et al. (1995) Characterization of cDNA and Genomic Clones Encoding Human Myelin Oligodendrocyte Glycoprotein. J Neurochem. 65(1): 309-18.
- Johns TG, et al. (1999) The Structure and Function of Myelin Oligodendrocyte Glycoprotein. J Neurochem. 72(1): 1-9.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"