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Product Name
Anti-MYC antibody
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Description
Mouse monoclonal to MYC
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Tested applications
WB
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Species reactivity
Recognize N-terminal, C-terminal in fusion proteins.
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Alternative names
MRTL antibody; MYCC antibody; c-Myc antibody; bHLHe39 antibody; Myc2 antibody; Nird antibody; Niard antibody; bHLHe39 antibody; AU016757 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1/5000-1/10000
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Validations
Anti-Myc Tag Antibody, Mouse MAb, Western blot
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Background
Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.
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References
- Ding X, et al., 2009, PLoS One. 4(6): e5949.
- Ratsima H, et al., 2011, Proc Natl Acad Sci. 108(43): E914-23.
- Chiang YJ, et al., 2013, PLoS One. 8(4): e61761.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"