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Product Name
Anti-mTOR Rabbit antibody
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Description
mTOR Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human mTOR
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/100
IP: 1/30
FC: 1/20
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Validations
Western blot analysis of mTOR expression in SHSY-5Y and Rat Brain cell lysates using mTOR antibody at 1/1000 dilution.Predicted band size:289KDa.Observed band size:289KDa.
Immunohistochemical analysis of paraffin-embedded rat liver, using mTOR Antibody.
Western blot analysis of mTOR expression in Jurkat cell lysate.
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Background
Swiss-Prot Acc.P42345.An atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.
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References
- Effect of metformin on human periodontal ligament stem cells cultured with polydopamine-templated hydroxyapatite
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"