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Product Name
Monomethyl auristatin E (MMAE) Monoclonal Antibody
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Description
Product has been tested in direct ELISA.
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Tested applications
ELISA
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Species reactivity
Chemical
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Isotype
Mouse IgG1, κ
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Preparation
MMAE conjugated with KLH. Protein G affinity purified
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Clonality
Monoclonal
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Formulation
PBS(pH7.4), lyophilized. Reconstitution: Adding sterile water, prepare a stock solution of 2.0 mg/ml.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
ELISA: 1:5,000-1:20,000
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Validations
ELISA Binding Assay of Anti-MMAE Antibody to different proteins.
ELISA analysis of Anti-MMAE Monoclonal Antibody with MMAE-BSA, MMAE ADC and BSA. Anti-MMAE Monoclonal Antibody can bind to MMAE-BSA or MMAE ADC but not carrier protein.
ELISA Binding Assay of Anti-MMAE Antibody
Immobilized MMAE ADC at 2μg/mL (100μL/well) can bind anti-MMAE mouse monoclonal antibody.
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Background
Auristatins are synthetic analogs of dolastatin 10 (D10), a highly cytotoxic antineoplastic agent employed for cancer chemotherapy, which is derived from a marine species known as Dolabella auricularia. Monomethyl auristatin E (MMAE) and monomethyl auristatin F (MMAF) are identified as the best analogs of dolastatin 10, which bind to microtubules and prevent cell proliferation by inhibiting mitosis. MMAE is one of the most commonly used payloads for developing antibody–drug conjugates (ADC).
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References
- Okeley NM, Miyamoto JB, Zhang X, Sanderson RJ, Benjamin DR, Sievers EL, Senter PD, Alley SC. Intracellular activation of SGN-35, a potent anti-CD30 antibody-drug conjugate. Clin Cancer Res. 2010 Feb 1;16(3):888-97. doi: 10.1158/1078-0432.CCR-09-2069. Epub 2010 Jan 19. Erratum in: Clin Cancer Res. 2011 Aug 15;17(16):5524. PMID: 20086002.
- Buckel L, Savariar EN, Crisp JL, Jones KA, Hicks AM, Scanderbeg DJ, Nguyen QT, Sicklick JK, Lowy AM, Tsien RY, Advani SJ. Tumor radiosensitization by monomethyl auristatin E: mechanism of action and targeted delivery. Cancer Res. 2015 Apr 1;75(7):1376-1387. doi: 10.1158/0008-5472.CAN-14-1931. Epub 2015 Feb 13. PMID: 25681274; PMCID: PMC4458508.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"