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Product Name
Anti-MICU1 antibody
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Description
Rabbit polyclonal antibody to MICU1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CALC antibody; EFHA3 antibody; MPXPS antibody; CBARA1 antibody; ara CALC antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was recombinant protein within human cbara1 aa 14-154.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB:1:500-1:2,000
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Validations
Fig1: Western blot analysis of CBARA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Mouse kidney tissue lysate; Lane 2: Rat brain tissue lysate; Lane 3: Hela cell lysate
- Background
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References
- Perocchi F et al. MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptake. Nature 467:291-296 (2010).
- Mallilankaraman K et al. MICU1 is an essential gatekeeper for MCU-mediated mitochondrial Ca(2+) uptake that regulates cell survival. Cell 151:630-644 (2012).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"