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Product Name
Anti-MARCHF7 antibody
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Description
Rabbit polyclonal antibody to MARCHF7
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
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Alternative names
AXO antibody; AXOT antibody; MARCH7 antibody; RNF177 antibody; MARCH-VII antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human march7/axotrophin 31-130/704
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Fig2: Sample:; Siha(Human) Cell Lysate at 30 ug; Primary: Anti-MARCH7 at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 78 kD; Observed band size: 73 kD
Fig3: Sample:; Siha(Human) Cell Lysate at 30 ug; U251(Human) Cell Lysate at 30 ug; Primary: Anti-MARCH7 at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 78 kD; Observed band size: 73 kD
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Fig5: Sample:; Siha(human)cell Lysate at 30 ug; MCF-7(human)cell Lysate at 30 ug; U251(human)cell Lysate at 30 ug; testis(rat) Lysate at 40 ug; Primary: Anti-MARCH7 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 78kD; Observed band size: 73 kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"