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Product Name
Anti-LSM1 antibody
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Description
Rabbit polyclonal to LSM1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human LSM1
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Alternative names
U6 small nuclear RNA associated (S. cerevisiae) antibody; U6 small nuclear RNA associated (S. cerevisiae) antibody; 2810025O06Rik antibody; cancer-associated Sm-like protein antibody; CASM antibody; CASM antibody; Lsm1 antibody; LSM1 antibody; LSM1 homolog antibody; LSM1 homolog antibody; small nuclear ribonuclear CaSm antibody; U6 snRNA-associated Sm-like protein LSm1 antibody; U6 snRNA-associated Sm-like protein LSm1 antibody; YJL124C antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human LSM1 (rh LSM1; O15116; Met1-Tyr133). LSM1 specific IgG was purified by Human LSM1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LSM1. The detection limit for Human LSM1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/ml
IP: 0.5-2 μg/mg of lysate
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Validations
LSM1 Antibody, Rabbit Pab, Antigen Affinity Purified, Western blot
LSM1 Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
LSM1 Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
LSM1 Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
LSM1 Antibody, Rabbit Pab, Antigen Affinity Purified, Immunoprecipitation
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Background
LSM1 is a Sm-like protein. Sm-like proteins can be detected in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins include the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM1 has a role in replication-dependent histone mRNA degradation and binds specifically to the 3''-terminal U-tract of U6 snRNA. LSM1 also facilitates RNA protein interactions and structural modifications which are required during ribosomal subunit assembly.
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References
- Shimizu Y, et al. (1997) Lineage- and differentiation stage-specific expression of LSM-1 (LPAP), a possible substrate for CD45, in human hematopoietic cells. Am J Hematol. 54(1):1=11.
- Graber MW, et al. (1997) CaSm: an Sm-like protein that contributes to the transformed state in cancer cells. Cancer Res. 57(14):2961-5.
- Séraphin B, et al. (1999) Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. EMBO J. 18(12):3451-62.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"