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Anti-LOC4344396 antibody

Cat.#: 175149

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Product Information

  • Product Name
    Anti-LOC4344396 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to LOC4344396
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Rice
  • Alternative names
    MTR1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within arogenate dehydratase aa 46-59.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:100-1:500

    IHC-P: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of Arogenate dehydratase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1: Western blot analysis of Arogenate dehydratase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Arogenate dehydratase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig2: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Arogenate dehydratase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Background
    Q6Z3Y3
  • References
    • Cho, Man-Ho; Corea, Oliver R. A.; Yang, Hong; Bedgar, Diana L.; Laskar, Dhrubojyoti D.; Anterola, Aldwin M.; et al. (2007). Phenylalanine biosynthesis in Arabidopsis thaliana-- identification and characterization of arogenate dehydratases. J. Biol. Chem. 282 (42): 30827–35.
    • Maeda, H.; Shasany, A. K.; Schnepp, J.; Orlova, I.; Taguchi, G.; Cooper, B. R.; et al. (2010). RNAi suppression of Arogenate Dehydratase 1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals. Plant Cell. 22 (3): 832–849.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"