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Product Name
Anti-LILRB3 antibody
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Description
Rabbit monoclonal to LILRB3
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Tested applications
IF, ICC/IF
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Species reactivity
Mouse LILRB3
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Alternative names
CD85A antibody; Lilrb3 antibody; LIR-3 antibody; Gp91 antibody; HL9 antibody; ILT5 antibody; LILRB3 antibody; LIR3 antibody; MGC138403 antibody; Pirb antibody; PIRB antibody; XXbac-BCX105G6.7 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LILRB3 (rM LILRB3; AAC53219.1; Met1-Tyr640).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 10-25 μg/mL
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Validations
LILRB3 Antibody, Rabbit MAb, Immunofluorescence
Immunofluorescence staining of mouse LILRB3 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse LILRB3 monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
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Background
Leukocyte immunoglobulin-like receptor subfamily B member 3, also known as Leukocyte immunoglobulin-like receptor 3, Immunoglobulin-like transcript 5, Monocyte inhibitory receptor HL9, CD85 antigen-like family member A, CD85a and LILRB3, is a single-pass type I membrane protein which belongs to the leukocyte receptor cluster (LRC) present on 19q13.4. LILRB3 / CD85a contains four Ig-like C2-type (immunoglobulin-like) domains. LILRB3 / CD85a contains three copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. LILRB3 / CD85a is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found.
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References
- Huang,J. et al., 2010, J Virol. 84 (18): 9463-71.
- Arm J.P. et al., 1997, J. Immunol. 159:2342-2349.
- Wende H. et al., 2000, Immunogenetics 51:703-713.
- Yu L.-R. et al., 2007,J. Proteome Res. 6:4150-4162.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"