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Product Name
Anti-Legumain Rabbit antibody
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Description
Legumain Rabbit monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
AEP; LGMN1; PRSC1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human Legumain
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
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Validations
Western blot detection of Legumain in Jurkat,Hela cell lysates using Legumain Rabbit mAb(1:1000 diluted).Predicted band size:49kDa.Observed band size:56,46,37kDa.
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Background
Swiss-Prot Acc.Q99538.Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation . May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"