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Product Name
Anti-LC3B (7B12) Mouse antibody
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Description
LC3B (7B12) Mouse monoclonal antibody
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Tested applications
WB, FC
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Species reactivity
Human, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human MAP1LC3B expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).
Western blot analysis of extracts from A549,MCF7,C6 and Hela cell lysates using LC3B mouse mAb (1:500 diluted).Predicted band size:14KDa.Observed band size:14,16KDa.
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Background
Swiss-Prot Acc.Q9GZQ8.The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"