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Product Name
Anti-KLF4 (7F8) Mouse antibody
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Description
KLF4 (7F8) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human KLF4 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Western blot detection of KLF4 in A549 and MCF7 cell lysates using KLF4 mouse mAb (1:2000 diluted).Predicted band size:54KDa.Observed band size:50KDa.
Immunofluorescence analysis of ECA109 cells using KLF4 mouse mAb (green). Red
Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) using KLF4 mouse mAb with DAB staining
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Background
Swiss-Prot Acc.O43474.Kruppel-like factor 4 (gut),endothelial Kruppel-like zinc finger protein. May act as a transcriptional activator. Binds the CACCC core sequence. May be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"