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Product Name
Anti-KDM4B/JMJD2B Rabbit antibody
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Description
KDM4B/JMJD2B Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
JMJD2B; TDRD14B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human KDM4B/JMJD2B
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/20
ICC/IF: 1/200
FC: 1/200
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Validations
Immunocytochemistry of KDM4B/JMJD2B(green) in Hela cells using KDM4B/JMJD2B Rabbit pAb at dilution 1/50, and DAPI(blue)
Immunohistochemistry of KDM4B/JMJD2B in paraffin-embedded Human lung cancer tissue using KDM4B/JMJD2B Rabbit pAb at dilution 1/20
Western blot detection of KDM4B in 293,COS7 cell lysates using KDM4B/JMJD2B Rabbit pAb(1:1000 diluted).Predicted band size:122KDa.Observed band size:150KDa.
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Background
Swiss-Prot Acc.O94953.Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"