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Product Name
Anti-Kcna4 antibody
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Description
Rabbit polyclonal antibody to Kcna4
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Tested applications
WB, IHC-P, ICC, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
Kv1. antibody; Kv1.4 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was peptide
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
IHC-P: 1:50-1:100
ICC: 1:50-1:200
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.
Fig2: ICC staining Kv1.4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Kv1.4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of SH-SY5Y cells with Kv1.4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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