Anti-ITPR2 antibody

Cat.#: 175135

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Product Information

  • Product Name
    Anti-ITPR2 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to ITPR2
  • Tested applications
    Dot blot, ICC, FC, IHC-P
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ANHD antibody; IP3R2 antibody; CFAP48 antibody; INSP3R2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein within human itpr2 aa 1100-1450.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    Dot Blot: 1:2,000-1:5,000

    ICC: 1:50-1:100

    IHC-P: 1:50 -1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

    Fig1: Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

    Fig2: ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Fig9: Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

    Fig9: Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

  • Background
  • References
    • Shi JL et al. High expression of inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) as a novel biomarker for worse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget 6(7):5299-5309(2015).
    • Zhang F et al. Genome-wide association study identifies ITPR2 as a susceptibility gene for Kashin-Beck disease in Han Chinese. Arthritis Rheumatol 67(1):176-181(2015).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"