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Product Name
Anti-ING5 antibody
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Description
Rabbit monoclonal to ING5
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Tested applications
ELISA, WB, IP
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Species reactivity
Human ING5
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Alternative names
1700001C14Rik antibody; 1700027H23Rik antibody; p28ING5 antibody; AI225768 antibody; 1700027H23Rik antibody; 1810018M11Rik antibody; AI225768 antibody; inhibitor of growth protein 5 antibody; inhibitor of growth protein 5 antibody; p28ING5 antibody; 1700001C14Rik antibody; 1810018M11Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ING5 (rh ING5; Q8WYH8-2; Glu36-Gln84).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human ING5.
IP: 1-4 uL/mg of lysate
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Validations
ING5 Antibody, Rabbit MAb, Western blot
ING5 Antibody, Rabbit MAb, Immunoprecipitation
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Background
ING5 belongs to the ING family. It contains 1 PHD-type zinc finger and is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16 and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2. ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. It can bind TP EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.
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References
- Zhang F, et al. (2011) The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects. PLoS One. (7):e21505.
- Zheng HC, et al. (2011) The nuclear to cytoplasmic shift of ING5 protein during colorectal carcinogenesis with their distinct links to pathologic behaviors of carcinomas. Hum Pathol. 42(3):424-33.
- Xing YN, et al. (2011) The altered expression of ING5 protein is involved in gastric carcinogenesis and subsequent progression. Hum Pathol. 42(1):25-35.
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