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Anti-ILDR1 antibody

Cat.#: 175524

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-ILDR1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to ILDR1
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep
  • Alternative names
    DFNB42 antibody; ILDR1beta antibody; ILDR1alpha antibody; ILDR1alpha' antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human ildr1 101-200/546
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:3ug/Test

  • Validations

    Fig1: Blank control: A431.; Primary Antibody (green line): Rabbit Anti-ILDR1 antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody: Goat anti-rabbit IgG-AF647; Dilution: 1μg /test.; Protocol; The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig1: Blank control: A431.; Primary Antibody (green line): Rabbit Anti-ILDR1 antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody: Goat anti-rabbit IgG-AF647; Dilution: 1μg /test.; Protocol; The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig2: Sample:; DU145(Human) Cell Lysate at 30 ug; Primary: Anti-ILDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 60 kD; Observed band size: 56 kD

    Fig2: Sample:; DU145(Human) Cell Lysate at 30 ug; Primary: Anti-ILDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 60 kD; Observed band size: 56 kD

    Fig3: Sample:; Prostate(Rat) Cell Lysate at 40 ug; Primary: Anti-ILDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 60 kD; Observed band size: 56 kD

    Fig3: Sample:; Prostate(Rat) Cell Lysate at 40 ug; Primary: Anti-ILDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 60 kD; Observed band size: 56 kD

    Fig4: Sample:; Pancreas(Mouse) Cell Lysate at 40 ug; Primary: Anti-ILDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 60 kD; Observed band size: 56kD

    Fig4: Sample:; Pancreas(Mouse) Cell Lysate at 40 ug; Primary: Anti-ILDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 60 kD; Observed band size: 56kD

  • Background
    Q86SU0

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"