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Anti-IL7 antibody

Cat.#: 175037

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Product Information

  • Product Name
    Anti-IL7 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to IL7
  • Tested applications
    ELISA, WB, ICC, IHC-P
  • Species reactivity
    Human, Rat
  • Alternative names
    IL-7 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.17% Sodium Azide.
  • Storage instructions
    Store at -20 ℃. Stable for 12 months from date of receipt.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:100-1:500

    IHC-P: 1:50-1:200

    ELISA: 1:5,000-1:10,000

  • Validations

    Fig1: Western blot analysis of IL7 on human thymus cell lysates using anti- IL7 antibody at 1/500 dilution.

    Fig1: Western blot analysis of IL7 on human thymus cell lysates using anti- IL7 antibody at 1/500 dilution.

    Fig2: ICC staining IL7 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining IL7 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining IL7 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining IL7 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining IL7 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining IL7 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: ICC staining IL7 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: ICC staining IL7 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL7 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL7 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL7 antibody. Counter stained with hematoxylin.

  • Background
    P13232
  • References
    • Ming, J. et al. 2011. Interleukin-7 up-regulates cyclin D1 via activator protein-1 to promote proliferation of cell in lung cancer. Cancer Immunol Immunother.
    • Zhang, Y. et al. 2015. Mutual enhancement of IL-2 and IL-7 on DNA vaccine immunogenicity mainly involves regulations on their receptor expression and receptor-expressing lymphocyte generation. Vaccine. 33: 3480-7.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"