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Product Name
Anti-IL32 antibody
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Description
Rabbit polyclonal to IL32
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Tested applications
ELISA, WB
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Species reactivity
Human IL32
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Alternative names
IL32 antibody; IL-32 alpha antibody; IL-32beta antibody; IL-32delta antibody; IL-32gamma antibody; NK4 antibody; TAIF antibody; TAIFa antibody; TAIFb antibody; TAIFc antibody; TAIFd antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human IL32 (rh IL32; NP_001012651.1; Met1-Lys131). IL32 specific IgG was purified by Human IL32 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL32. The detection limit for Human IL32 is < 0.039 ng/well.
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Validations
IL32 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines, and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma and delta) are the most representative IL-32 transcripts, and gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis and in human stomach cancer, human lung cancer and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.
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References
- Felaco P, et al. (2009) IL-32: a newly-discovered proinflammatory cytokine. J Biol Regul Homeost Agents. 23(3): 141-7.
- Kobayashi H, et al. (2009) Molecular characterization of IL-32 in human endothelial cells. Cytokine. 46(3): 351-8.
- Meyer N, et al. (2010) IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol. 125(4): 858-865.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"