Anti-IL31 antibody

Cat.#: 175033

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Product Information

  • Product Name
    Anti-IL31 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to IL31
  • Tested applications
    WB, IHC-P, ICC
  • Species reactivity
    Human
  • Alternative names
    IL-31 antibody
  • Isotype
    Mouse IgG3
  • Preparation
    This antigen of this antibody was recombinant full length protein corresponding to human il18.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500

    ICC: 1:100

    IHC-P: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.

    Fig1: Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.

    Fig2: ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-31 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-31 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-31 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-31 antibody. Counter stained with hematoxylin.

  • Background
  • References
    • Dillon S R et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat Immunol 5:752-760 (2004).
    • Neis M M et al. Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis. J Allergy Clin Immunol 118:930-937(2006).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"