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Product Name
Anti-IL1RAPL1 antibody
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Description
Mouse monoclonal antibody to IL1RAPL1
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Tested applications
WB, ICC, FC
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Species reactivity
Human
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Alternative names
IL1R8 antibody; MRX10 antibody; MRX21 antibody; MRX34 antibody; OPHN4 antibody; IL1RAPL antibody; TIGIRR-2 antibody; IL1RAPL-1 antibody; IL-1RAPL-1 antibody; IL-1-RAPL-1 antibody
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:100-1:200
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Validations
Fig1: Western blot analysis of IL1RAPL1 on human IL1RAPL1 recombinant protein using anti-IL1RAPL1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of IL1RAPL1 on HEK293 (1) and IL1RAPL1-hIgGFc transfected HEK293 (2) cell lysate using anti-IL1RAPL1 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of IL1RAPL1 on different cell lysate using anti-IL1RAPL1 antibody at 1/1,000 dilution.; Positive control:; Lane 1: A431; Lane 2: SK-Hep-1; Lane 3: HL-7702
Fig4: ICC staining IL1RAPL1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with IL1RAPL1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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References
- Montani C et al. The X-Linked Intellectual Disability Protein IL1RAPL1 Regulates Dendrite Complexity.J Neurosci 37(28):6606-6627 (2017).
- Laino L et al. Clinical and molecular characterization of a boy with intellectual disability, facial dysmorphism, minor digital anomalies and a complex IL1RAPL1 intragenic rearrangement.Eur J Paediatr Neurol 20(6):971-976 (2015).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"