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Product Name
Anti-IL-3 antibody
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Description
Rabbit polyclonal to IL-3
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Tested applications
ELISA, IHC-P
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Species reactivity
Rat IL-3
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Alternative names
BPA antibody; PSF antibody; HCGF antibody; Il-3 antibody; MCGF antibody; Csfmu antibody; IL-3 antibody; MCGF antibody; MULTI-CSF antibody; Csfmu antibody; IL3 antibody; IL-3 antibody; MCGF antibody; MCGF antibody; MGC79398 antibody; PSF antibody; RP23-309E16.5 antibody; BPA antibody; HCGF antibody; Il3 antibody; IL-3 antibody; MGC79399 antibody; MULTI-CSF antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat IL-3 (rR IL-3; NP_113701.1; Met1-Cys169). IL-3 specific IgG was purified by Rat IL-3 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat IL-3. The detection limit for Rat IL-3 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
IL-3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of rat IL3 in rat spleen with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
IL3 (interleukin 3), also known as IL-3, is a potent growth promoting cytokine which belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
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References
- Meyer CG, et al.. (2011) IL3 variant on chromosomal region 5q31-33 and protection from recurrent malaria attacks. Hum Mol Genet. 20(6):1173-81.
- Zambrano A, et al. (2010) IL-3 controls tau modifications and protects cortical neurons from neurodegeneration. Curr Alzheimer Res. 7(7):615-24.
- Dentelli P, et al.. (2011) IL-3 is a novel target to interfere with tumor vasculature. Oncogene. 30(50):4930-40.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"